Volume 8 Issue 4 | April 2014 issue
Table of Contents
8(4) 2014

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Australian Journal of Crop Science | April 2014

Volume 8 Number 4  2014



Inheritance and QTL analysis of flour falling number using recombinant inbred lines derived from strong gluten wheat ‘Gaocheng 8901’ and waxy wheat ‘Nuomai 1’

Zhiying Deng, Fang Chen, Shuna Hu, Qingdian Han, Jiansheng Chen, Cailing Sun, Yongxiang Zhang, Shouyi Wang, Xuejiao Song, Jichun Tian*

State Key Laboratory of Crop Biology, Key Laboratory of Crop Biology of Shandong Province, Group of Wheat Quality Breeding, Shandong Agricultural University, Taian, Shandong, P.R. China, 271018
College of life science, Linyi University, Linyi, Shandong, P.R. China, 276005

Summary
Flour falling number (FN) is an important trait because not only related with pre-harvest sprouting (PHS) tolerance but also with processing quality. Waxy wheat usually has low flour falling number (FN) without severe pre-harvest sprouting (PHS). To identify quantitative trait loci (QTLs) associated with flour FN, a recombinant inbred line (RIL) population derived from two Chinese wheat, strong gluten wheat ‘Gaocheng 8901’ and waxy wheat ‘Nuomai 1’, was employed to evaluate the genetic variation and to detect QTLs based on DarT and SSR markers under three environments.

Pages 468-474 | Full Text PDF

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Determination of Chokanan mango sweetness (Mangifera indica) using non-destructive image processing technique

Siti Khairunniza Bejo* and Syahidah Kamaruddin

Department of Biological and Agricultural Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Summary
The Chokanan mango (Mangifera indica) has a high commercial potential. Its sugar content increases as the colour changes during the maturation process.  In this research, the relationship between the sweetness of the Chokanan mango and its mean pixel values in RGB and HSB colour space is analyzed.

Pages 475-480 | Full Text PDF

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Evaluation of genetic relationship among some important Japanese and Thai soybean varieties using AFLP analysis

Aungkana Nimnual, Jutamas Romkaew, Ekachai Chukeatirote, and Somrudee Nilthong*

School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand

Summary

Amplified fragment length polymorphism (AFLP) analysis was used to study the genetic relationship among 25 soybean varieties from Japan and Thailand.  Seventeen AFLP primer pairs produced 317 polymorphic (44.90%) and 389 (55.10%) monomorphic AFLP fragments.  The polymorphic information content (PIC) among varieties varied from 0.0357 (E-AAC/M-CAT) to 0.1464 (E-ACC/M-CTA) with an average of 0.1088.

Pages 481-485 | Full Text PDF

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Near-lethal heat treatment induced metabolic changes associated with endodormancy release of Superior Seedless grapevine cv. (Vitis vinifera L.) buds

Hatem Ben Mohamed*, Ahlem Zrig, Jan M.C. Geuns, Habib Khemira

Laboratory of Horticulture, National Agricultural Research Institute of Tunisia (INRAT), Rue Hιdi Karray 2049 Ariana, Tunisia
Research Unit Biodiversity and Valorization of Bioresources in Arid Zones, Faculty of Sciences of Gabθs, University of Gabθs, Erriadh City, Zrig 6072 Gabθs, Tunisia
Laboratory of Functional Biology, KU leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium
Centre for Environmental Research & Studies, Jazan University, Jazan, Saudi Arabia

Summary

To assess the efficacy of near lethal heat stress for endodormancy release of Superior Seedless grapevine buds, single-node cuttings were soaked for 1 h in hot water (50°C) and then forced for one month. The effects of hot water treatment (HWT) on budburst, metabolic changes of carbohydrates, proline, free polyamines PAs [putrescine (Put), spermidine (Spd) and spermine (Spm)] and antioxidant enzymes’ activity were investigated in bud under forcing conditions. Near-lethal heat stress caused a transient increase in starch hydrolysis, leading to an abrupt accumulation of soluble sugars, especially sucrose concentration during the first 3 days after treatment.

Pages 486-494 | Full Text PDF

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Effects of crop evapotranspiration estimation techniques and weather parameters on rice crop water requirement

M. M* Maina, M.S.M. Amin, Md. K. Rowshon, W. Aimrun, A. A. Samsuzana, and  M.A. Yazid


Department of Biological and Agricultural Engineering, Faculty of Engineering, UPM, Serdang, Malaysia
Smart Farming Technology Research Center, Faculty of Engineering,Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia
Irrigation and Agricultural Drainage Division, Ministry of Agriculture and Agro-based Industry, Malaysia

Summary

Accurate estimation of crop evapotranspiration is a key to determination of crop water requirements as well as water productivity. In this study, Microflex-C ultrasonic sensor was mounted on three sets of lysimeter to measure crop evapotranspiration in a paddy field of the Tanjung Karang Rice Irrigation Scheme. Evapotranspiration was obtained from water level recorded at 15 minutes interval for the entire irrigation season.

Pages 495-501 | Full Text PDF

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Study of genetic relationship among Kenyan cultivated potato clones using SSR markers

Jane Muthoni*, Hussein Shimelis, Rob Melis

African Centre for Crop Improvement, University of KwaZulu-Natal, College of Agriculture, Engineering and Science, School of Agricultural, Earth and Environmental Sciences, Private Bag X01, Scottsxille 3209, Pietermaritzburg, South Africa

Kenya Agricultural Research Institute (KARI). National Potato Research Centre, Tigoni, Kenya

Summary

The objective of this study was to determine the genetic relationships among Kenyan potato clones so as to complement other bacterial wilt-resistance data in identifying parents for a breeding program. Twenty potato clones were genotyped with twenty four SSR primer pairs selected for high polymorphism. The twenty four SSR primers identified 160 alleles among the 20 potato clones. The average number of alleles per locus ranged from 2 to 14 with an average of 6.67.

Pages 502-508 | Full Text PDF

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Morphological and anatomical investigation on endemic Cota melanoloma and Cota antitaurica (Asteraceae) in Turkey

Mehmet Ufuk Φzbek*, Dudu Φzlem Mavi Idman, Baris Bani

Gazi University, Faculty of Sciences, Department of Biology, 06500, Teknikokullar, Ankara, Turkey
Middle East Technical University, Department of Biological Sciences, 06531, Ankara, Turkey
Kastamonu University, Faculty of Arts and Sciences, Department of Biology, 37200, Kuzeykent, Kastamonu, Turkey

Summary
Anthemis section Cota was accepted as separate genus recently. Genus Cota is represented by 24 taxa in Turkey. Some of them have economic importance because of their uses for various purposes such as obtaining drugs, food and dye. etc. In this study, Cota melanoloma subsp. melanoloma, C. melanoloma subsp. trapezuntica and C. antitaurica were studied morphologically and anatomically. Three of them are endemic to Turkey. C. antitaurica is only known from the type locality and critically endangered species, while C. melanoloma is distributed in only northern Anatolia.
 
Pages 509-514 | Full Text PDF

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Assessing genetic variation for heat tolerance in synthetic wheat lines using phenotypic data and molecular markers

Pradeep Sharma*, Sindhu Sareen, Manoj Saini, Ajay Verma, Bhudev Singh Tyagi, Indu Sharma

Plant Biotechnology, Directorate of Wheat Research, Karnal 132001, Haryana, India
Crop Improvement Division, Directorate of Wheat Research, Karnal 132001, Haryana, India    
Computer Section, Directorate of Wheat Research, Karnal 132001, Haryana, India

Summary

High temperature is a major environmental stress factor limiting wheat productivity in India. Therefore, improvement for heat tolerance in wheat is an important breeding objective. A number of synthetic wheat hexaploids have shown resistance to major wheat diseases and tolerance to abiotic stresses such as drought, waterlogging and salinity. This study was conduct to assess heat tolerance and genetic variation among twenty-four heat tolerant synthetic wheat lines in order to identify new sources of diversity that could accelerate the development of improved wheat genotypes better suited to meet the challenges posed by changing climate in India.

Pages 515-522 | Full Text PDF

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Natural variation in response to nitrate starvation among varieties of habanero pepper (Capsicum chinense Jacq.)

Gladys Santiago-Antonio, Marta Guadalupe Lizama-Gasca, Mildred Carrillo-Pech, Ileana Echevarrνa-Machado*

Unidad de Bioquνmica y Biologνa Molecular de Plantas, Centro de Investigaciσn Cientνfica de Yucatαn (CICY), Calle 43 # 130, Col. Chuburnα de Hidalgo, 97200, Mιrida, Yucatαn, Mιxico

Summary

Habanero pepper (Capsicum chinense Jacq.) is one of the most cultivated plants in the world. We evaluated the physiological and molecular behavior of three habanero pepper varieties seedlings, two from the Yucatan region (Mayan Ek and Mayan Chan) and one commercial variety (SEMINIS), subjected to 10 days of nitrogen (N) deficiency treatment. Growth parameters, N compounds, net nitrate absorption, and CcNRT2.1 (Capsicum chinense high affinity nitrate transporter) gene expression were evaluated at 0, 2, 6, 8 and 10 days of the treatment.

Pages 523-535 | Full Text PDF

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Screening of rice varieties for direct seeding method

Muhammad Kamrul Islam, Md. Shahedul Islam, Jiban Krishna Biswas, Si Young Lee, Iftekhar Alam, Moo Ryong Huh*

Department of Horticulture, College of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Korea
Gana Unnayan Kendra (GUK), Nashratpur, Gaibandha 5700, Bangladesh
Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh
Division of Applied Life Science (BK21), College of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Korea

Institute of Agriculture & Life Science (IALS), Gyeongsang National University, Jinju 660-701, Korea

Summary
Direct seeding method of rice planting offers several advantages. However, all rice cultivars are not suitable for this practice. In this report forty-three high yield rice varieties and an anoxia/hypoxia tolerant control land race were studied to verify their suitability to establish seedling under anaerobic conditions.

Pages 536-542 | Full Text PDF

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Morphological, anatomical, pollen and seed morphological properties of Melilotus bicolor Boiss. & Balansa (Fabaceae) endemic to Turkey

Funda Φzbek*, Mehmet Ufuk Φzbek, Murat Ekici

Department of Biology, Faculty of Science, Gazi University, 06500 Ankara, Turkey

Summary
The genus Melilotus L. has a worldwide distribution and usually grows in the calcareous soil regions of the world. It is important for its use in folk medicine, as a forage crop and for green manure. The morphology, anatomy, pollen and seed morphology of Melilotus bicolor Boiss. & Balansa, which is an endemic species in Turkey, were studied. An expanded and amended description of the species collected from Beypazari to Kibriscik in Ankara is given.

Pages 543-549 | Full Text PDF

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Performance of winter wheat under different irrigation regimes associated with weather conditions in the North China Plain

Suying Chen, Hongyong Sun, Liwei Shao, Xiying Zhang*

Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang 050021, Hebei, China

Summary
The North China Plain (NCP) is one of the most important regions for grain production in China. However, its agricultural system is being significantly affected by ongoing climate change and is becoming vulnerable with water shortage. This study investigated the responses of wheat production under six irrigation treatments (from rain-fed up to five irrigations) during 2007 to 2012.

Pages 550-557 | Full Text PDF

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Molecular isolation and characterization of the Rac GTPase gene from Oryza sativa indica cultivar UKMRC9

Kalaivani Nadarajah*, Nurfarahana Syuhada Omar and Hazeq Sukri

School of Environmental Sciences and Natural Resource, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Malaysia
School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Malaysia

Summary

Rac GTPase is involved in the transduction and regulation of various cellular processes in eukaryotes such as the plant defense system. This regulatory gene has been reported to produce a broad spectrum resistance against pathogens in plants. The purpose of this study is to isolate and characterize the Rac GTPase gene from UKMRC9, a locally developed cultivar that shows tolerance to major rice diseases in Malaysia.

Pages 558-567 | Full Text PDF | Supplementary data
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Response of nitrate transporters and PM H+-ATPase expression to nitrogen flush on two upland rice varieties contrasting in nitrate uptake kinetics

Marcus Vinνcius Loss Sperandio, Leandro Azevedo Santos, Osmαrio Josι Lima de Araϊjo, Renan Pinto Braga, Cαssia Pereira Coelho, Eduardo de Matos Nogueira, Manlio Silvestre Fernandes, Sonia Regina de Souza*

Universidade Federal Rural do Rio de Janeiro, Departamento de Solos, Instituto de Agronomia, Rodovia BR 465 Km 7 Seropιdica RJ 23890-000, Brazi
Universidade Federal do Estado do Rio de Janeiro, Departamento de Genιtica e Biologia Molecular, Rua Frei Caneca 94 Centro RJ 20211-040, Brazil
Universidade Federal Rural do Rio de Janeiro, Departamento de Quνmica, Instituto de Ciκncias Exatas, Rodovia BR 465 Km 7 Seropιdica RJ 23890-000, Brazil

Summary

The aim of this study was to evaluate the behavior of high-affinity nitrate (NO3-) transporters and plasma membrane H+-ATPase isoforms in rice varieties, contrasting in NO3- uptake kinetics parameters. Two varieties of rice were studied: cv. IAC-47 (improved for high N input) and cv. Piauν (a local landrace variety cultivated for low input farmers). We evaluated the expression of two PM H+-ATPase isoforms (OsA2 and OsA7), two NO3- transporters (OsNRT2.1 and OsNRT2.2), and OsNAR2.1, the rice regulatory genes for nitrate transport.

Pages 568-576 | Full Text PDF

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Organic amendments and nitrogen effects on growth and chemical composition of two cultivars of safflower (Carthamus tinctorius L.)

Ruhollah Naderi and Ehsan Bijanzadeh

Department of Agroecology, College of Agriculture and Natural Resources of Darab, Shiraz University, Iran

Summary

Recently, public concern has been raised about potential environmental pollution caused by excessive use of chemical fertilizers. A greenhouse experiment was conducted to evaluate the effects of municipal waste compost (MWC), vermicompost (VC), cow manure (CM), sheep manure (SM) and nitrogen (N) on the growth and chemical composition of safflower in view as substitutes of fertilizers.

Pages 577-581 | Full Text PDF

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Micro-irrigation and fertigation improves gas exchange, productivity traits and economics of Indian mustard (Brassica juncea L. Czernj and Cosson) under semi-arid conditions

Sanjay Singh Rathore, Kapila Shekhawat, Basant Kumar Kandpal and Om Prakash Premi

Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan, India 321303

Summary

An experiment was conducted to evaluate the effect of micro-sprinkler and drip irrigation with nitrogen fertigation on gas exchange traits, growth, yield and economics of Indian mustard during 2009-10 and 2010-11 under semi-arid conditions of Rajasthan, India. The experiment was conducted in split plot design, consisting of five irrigation systems in main plot viz. MS, MS+CB, DS, DS+CB and CB alone. Four nitrogen levels (N) 0, 40, 80 and 120 kg ha-1 were taken in the sub plots. The results revealed that MS and DS alone or in combination with CB influenced the physiological parameters including chlorophyll concentration, PAR, ICC, photosynthesis, transpiration and stomatal conductance.

Pages 582-595 | Full Text PDF

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Effect of selenium and silicon on yield quality of rice plant grown under drought stress

Manal M. Emam*, Hemmat E. Khattab, Nesma M. Helal, Abdelsalam Deraz

Botany Department, Faculty of Sciences, Ain Shams University, Abbassyia 11566, Cairo, Egypt
Agriculture Research Center, Rice Research Institute, Giza, Egypt


Summary
Improving the nutritional quality of food is an important step in addressing worldwide health problems. A field experiment was conducted to evaluate the effects of either Se (0.03mM sodium selenate) or Si (1.5mM potassium silicate ) on the yield components as well as grain and straw qualities of rice cv. Giza 177 and cv. IET 1444 exposed to water deficit condition. Drought stress was imposed by withholding water (irrigation every 10 days). The plants were left to grow under the various treatments for five months. At full maturity (5 months after sowing) the experiment was terminated and grain quality in terms of grain appearance (grain length L, grain breadth B and L/B ratio), cooking quality (amylose content and water uptake WU) as well as nutritional (carbohydrates, proteins oil, phenols and flavonoid contents) and anti-nutritional (phytic and oxalic acids) values was estimated.

Pages 596-605 | Full Text PDF

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Proteome profiling of seed from inbred and mutant line of Sorghum (Sorghum bicolor)

Swapan Kumar Roy, Abu Hena Mostafa Kamal, Hye-Rim Kim, Soo-Jeong Kwon, Hee-Young Jang, Jung-Hee Ko, Jong-in Kim, Tae-Seok Ko, Zhanguo Xin and Sun-Hee Woo*

Deptartment of Crop Science, Chungbuk National University, Cheongju 361-763, Korea
Functional Cereal Crop Research Div. National Institute of Crop Science, Miryang 627-803, Korea
School of Plant Life & Environmental Science, Hankyong National University, Anseong 456-749, Korea
Plant Stress and Germplasm Development Unit, USDA-ARS, 3810 4th Street, Lubbock, TX 79415, USA

Summary

Grain sorghum is a major staple food, with ?fth rank among the cereals world-wide, considering its importance for food and feed applications. Cereals are main part of human nutrition and strategic resources. In this study, we executed a comprehensive proteomic study to investigate the seed storage proteins using mature seeds from inbred line (BTx623) and mutant line (M2P1207) of sorghum.

Pages 606-611 | Full Text PDF

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Effect of nanoparticles on biological contamination of in vitro cultures and organogenic regeneration of banana

Mohamed Naser Helaly, Mohamed Ahmed El-Metwally, Hanan El-Hoseiny, Samar Abdelaziz Omar, Nabil Ibrahim El-Sheery*  

Agricultural Botany Department, Faculty of Agriculture, Mansoura University, El-Mansoura, Egypt 35516
Mycological Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Horticultural Research Institute, Agricultural Research Center, Giza, Egypt
Genetic department, Faculty of Agriculture, Tanta University, Egypt
Agricultural Botany Department, Faculty of Agriculture, Tanta University, Tanta, Egypt

Summary
This study aimed to identify biological contaminants of banana in vitro cultures. We also tested the effect of Zn or ZnO nanoparticles on elimination of some bacterial and fungal contaminants and their influence regeneration. Nine strains of bacterial contaminants (Cellulomonas uda, Cellulomonas flarigena, Corynebacterium panrometabolum, Bacillus megaterium, Staphylococcus spp., Klebsiella spp., Erwinia cypripedii, Pseudomonas spp. and Proteus spp.) and four fungal (Fusarium spp., Aspergillus spp., Penicillium spp. and Candida spp.) contaminants were identified in nanoparticles-free media of banana in vitro cultures.

Pages 612-624 | Full Text PDF

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Usefulness of RAPD, RFLP and SCAR molecular markers and AGPaseB gene methylation level in the screening of resistance to the golden cyst nematode (Globodera rostochiensis) pathotype Ro1 in different Polish potato genotypes

Michal RUREK*, Renata Anna GALEK, Malgorzata JAKUBOWICZ, Grzegorz PIETKIEWICZ, Leszek STYSZKO, Ewa Janina SAWICKA- SIENKIEWICZ

Department of Molecular and Cellular Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland
Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 24a, 53-363 Wroclaw, Poland,
Department of Biotechnology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland
Department of Civil and Environmental Engineering, Division of Environmental Biology, Koszalin University of Technology, Sniadeckich 2, 75-453 Koszalin, Poland

Summary
The aim of this study was to assess the application of various DNA markers as well as the ADP-glucose pyrophosphorylase small subunit (AGPaseB) gene methylation for the screening of potato cultivars and breeding lines with different resistance to Globodera rostochiensis. Tetraploid genotypes were also included. The 2 kb and 0.7 kb random amplified polymorphic (RAPD) fragments as well as a PCR-amplified 1.6 kb fragment of AGPaseB gene positioned close to the Gro1 locus were tested. Two novel sequence characterised amplified region (SCAR) fragments were generated from 2 kb RAPD product of susceptible and resistant genotypes.

Pages 625-630 | Full Text PDF | Supplementary data

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Exogenous salicylic acid alleviates salt stress-induced oxidative damage in Brassica napus by enhancing the antioxidant defense and glyoxalase systems

Mirza Hasanuzzaman*, Md. Mahabub Alam1, Kamrun Nahar, Jubayer-Al-Mahmud, Kamal Uddin Ahamed and Masayuki Fujita

Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan
Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh
Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh
Department of Agroforestry and Environmental Science, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh

Summary
Regulatory roles of exogenous salicylic acid (SA) on the antioxidant defense and methylglyoxal (MG) detoxification systems were investigated in rapeseed seedlings (Brassica napus L. cv. BINA Sharisha 3) grown under salinity. Ten-day-old seedlings, grown in petri dishes, were supplemented with SA and salt (100 and 200 mM NaCl) separately and in combination for 48 h. After treatment, MDA and H2O2 content, non-enzymatic and enzymatic components of antioxidant and glyoxalase enzymes were measured.

Pages 631-639 | Full Text PDF

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The usefulness of EM-AMMI to study the influence of missing data pattern and application to Polish post-registration winter wheat data

Jakub Paderewski and Paulo Canas Rodrigues

Department of Experimental Design and Bioinformatics, Warsaw Univ. of Life Sciences, Nowoursynowska 159, 02-766 Warsaw, Poland
CMA-Center for Mathematics and Applications, Faculty of Sciences and Technology, Nova University of Lisbon, 2829-516 Caparica, Portugal
Universidade Europeia, Laureate International Universities, Lisbon, Portugal

Summary

In this paper we present a simulation study to investigate the influence of the pattern of missing values on the efficiency of the expectation-maximization AMMI (EM-AMMI) algorithm. Four scenarios are considered: one with cells missing completely at random; and three patterns with cells not missing at random (block-diagonal pattern, diagonal pattern and block-diagonal pattern with checks). The results are compared in terms of precision to estimate the missing cells and genotype selection.

Pages 640-645 | Full Text PDF | Supplementary data

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April 2014 | Australian Journal od Crop Science