Volume 8 Issue 1 | January 2014 issue
Table of Contents
8(1) 2014

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Australian Journal of Crop Science | January 2014

Volume 8 Number 1  2014



Effects of salinity on expression of the salt overly sensitive genes in Aeluropus lagopoides

Masoomeh Jannesar, Khadije Razavi*, Azra Saboora

National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran
National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e Pajoohesh, 17th Km of Tehran-Karaj Highway, P.O.Box: 14965/161, Tehran, I.R.Iran

Summary
In this study, seeds of Aeluropus lagopoides were cultured in hydroponic medium supplemented by ½MS medium. After 21 days, seedlings were treated with NaCl (600 mM), ABA (abscisic acid) (50 ΅M), Ca2SO4 (5 mM), NaCl+Ca2SO4, NaCl+ABA and Ca2SO4+ABA. Expression analysis of the A. lagopoides salt overly sensitive genes (SOS1, SOS2 and SOS3) was done by Semi-Quantitative RT-PCR. 

Pages 1-8 | Full Text PDF
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Genetic diversity of lemba (Curculigo latifolia) populations in Peninsular Malaysia using ISSR molecular markers

Ali Ranjbarfard, Ghizan Saleh*, Nur Ashikin Psyquay Abdullah and Pedram Kashiani

Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Summary
Genetic diversity of 45 populations of lemba (Curculigo latifolia) collected from various ecotypes of Peninsular Malaysia was analyzed using ISSR markers. Seven primers were found to produce polymorphic and reproducible bands.

Pages 9-17 | Full Text PDF

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Review article

Field performance of Bt transgenic crops: A review

Fei Wang, Shaobing Peng, Kehui Cui, Lixiao Nie, Jianliang Huang*

National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China

Summary
Here this review summarizes the researches on the field performance of Bt transgenic crops relative to their non-Bt counterparts by focusing on differences in agronomic traits and Bt protein content, and the effect of abiotic stresses on the performance of Bt transgenic crops. Furthermore, we discussed the physiological mechanisms underlying the variation of Bt protein content at different growth stages, or due to various environmental factors.

Pages 18-26 | Full Text PDF

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Screening of soybean [Glycine max (L.) Merr.] genotypes for yellow mosaic virus (YMV) disease resistance and their molecular characterization using RGA and SSRs markers

Bhupender Kumar, Akshay Talukdar*, Khushbu Verma, Vanishree Girmilla, Indu Bala, Sanjay Kumar Lal, Kanwar Pal Singh, Ramesh Lal Sapra

Cummings’s Lab., Directorate of Maize Research, Pusa Campus New Delhi-12, India
Division of Genetics, Indian Agricultural Research Institute, New Delhi-12, India

Directorate of Sorghum Research, Hyderabad, India
Molecular Cytogenetics and Tissue Culture Lab., Department of Crop Improvement, CSK Himachal Pradesh Agricultural University, Palampur 176 062, India

Summary
The present study was conducted to identify stable sources of resistance for YMV disease, and their molecular characterization. A 500 soybean germplasm lines, collected from different parts of the world were screened for YMV disease reaction at YMV hotspot consecutively for 3 years
.

Pages 27-34 | Full Text PDF | Supplementary data PDF
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Genetic diversity in ex-situ conserved sorghum accessions of Botswana as estimated by microsatellite markers

Tiny Motlhaodi*, Mulatu Geleta, Tomas Bryngelsson, Moneim Fatih, Stephen Chite, Rodomiro Ortiz

Swedish University of Agricultural Sciences, Department of Plant Breeding, P. O. Box 101, SE-230 53 Alnarp, Sweden
Department of Agricultural Research, Private Bag 0033, Gaborone, Botswana
Swedish Biodiversity Centre, P. O. Box 91, Pomonavδgen 2, SE-230 53 Alnarp, Sweden

Summary
Simple sequence repeat (SSR) markers were used to characterize genetic diversity in 30 sorghum [Sorghum bicolor (L.) Moench] accessions conserved in the national gene bank of Botswana. This collection represents the three sorghum-growing agro-ecological zones in the country.

Pages 35-43 | Full Text PDF
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Isolation and characterization of Dehydration-Responsive Element-Binding Factor 2 (DREB2) from Indian wheat (Triticum aestivum L.) cultivars

Bharati Pandey, Pradeep Sharma*, Manoj Saini, Dev Mani Pandey, Indu Sharma

Plant Biotechnology, Directorate of Wheat Research, Karnal-132001, India
Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi- 835 215, India

Summary
Dehydration responsive element binding (DREB) proteins are important transcription factors known to regulate diverse processes of plant development and stress responses. In this study, DREB2 gene was amplified using specific primer from a diverse set of bread wheat cultivars.


Pages 44-54 | Full Text PDF | Supplementary data PDF
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SSR based genetic diversity analysis in a diverse germplasm of groundnut (Arachis hypogaea L.) from Pakistan

Sohaib Roomi*, Bibi Sabiha, Arshad Iqbal, Muhammad Suleman, Izhar Muhammad, Muhammad Amir Zia, Muhammad Zulfiqar Ahmad, Farooq Rashid, Abdul Ghafoor and Nabila Tabbasam

Department of Biosciences COMSATS Institute of Information Technology, Islamabad, Pakistan
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology, Islamabad, Pakistan
Institute of Biotechnology and Microbiology, University of Swat, Khyber Pakhtunkhwa, Pakistan
National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Centre, Islamabad, Pakistan

Summary
The current study was aimed to explore the genetic diversity among seventy Pakistani accessions of Arachis hypogea. In Pakistan their morphological and biochemical variations have already documented but still so for, molecular variations need to be studied for this valuable crop.


Pages 55-61 | Full Text PDF

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Comparative changes in the rheological properties and cell wall metabolism in rind of healthy and creased fruit of Washington Navel and Navelina sweet orange (Citrus sinensis [L.] Osbeck)

Basharat Ali Saleem, Imran Hassan, Zora Singh, Aman Ullah Malik, Muhammad Aslam Pervez

Curtin Horticulture Research Laboratory, Department of Environment and Agriculture, School of Science and International Institute of Agri-Food Security (IIAFS), Curtin University, GPO Box U1987, Perth, 6845, WA, Australia

Summary
Creasing is a physiological disorder in navel oranges and causes serious economic losses. In 2007,  the comparative changes in rheological properties of the rind,  levels of  starch, phenolics, pectins and the activity of pectinesterase (PE) in albedo and flavedo tissues of the healthy and the creased fruit of ‘Washington Navel’ and ‘Navelina’ sweet orange at ripe  stage were investigated.

Pages 62-70 | Full Text PDF

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Influence of foliarly applied different triazole compounds on growth, nutrition, and antioxidant enzyme activities in tomato (Solanum lycopersicum L.) under salt stress

Atilla Levent Tuna

Department of Biology, Faculty of Science, Mugla University, Mugla, Turkey

Summary
A pot experiment was conducted to investigate the effects of five different triazole compounds, triadimenol, tebuconazole, bitertanol, triadimefon,  and paclobutrazol  on the growth, macro-nutrition, antioxidative enzyme activities and other stress related parameters in Solanum lycopersicum L. (tomato) plants grown in greenhouse conditions under salt stress.

Pages 71
-79 | Full Text PDF
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Spatial and temporal salinity accumulation patterns on golf course fairway soils under effluent water irrigation

David J. Skiles, Saad F. Alshammary, and Yaling Qian*

Deptartment of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523-1173, USA
National Center of Agriculture Technology, King Abdulaziz City for Science & Technology, Riyadh, Saudi Arabia

Summary
In this study, salinity accumulation patterns on 4 fairways of two golf courses irrigated with effluent water were investigated using two different types of sensors. Spatial and temporal salt accumulation patterns were measured using a network of in-situ soil sensors located at two depths (15 and 30 cm for 5TE sensor and 8 and 19cm for Turf Guard sensor TG2).

Pages 80-90 | Full Text PDF

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Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions

Mina Ghahremani, Faezeh Ghanati*, Francoise Bernard, Taha Azad, Morteza Gholami, Masoumeh Safari

Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University, POB 14115-154, Tehran, Iran
Department of Plant Biology, Faculty of Biological Science, Shahid Beheshti University, Tehran, Iran
Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Medicinal Plants and Drugs Research Institute (MPDRI), Shahid Beheshti University, G.C., Tehran, Iran

Summary
The present study was undertaken to evaluate the impact of ornithine, as the common source of proline and polyamines, in the biosynthesis of proline and polyamines and in the activation of antioxidant enzymes in the tobacco cells under saline conditions.


Pages 91-96 | Full Text PDF
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Genetic diversity of chilli (Capsicum annuum L.) genotypes of India based on morpho-chemical traits

Tasso Yatung, Rakesh Kr. Dubey*, Vikas Singh and Garima Upadhyay

Department of Vegetable Science, College of Horticulture & Forestry, CAU, Pasighat Arunachal Pradesh, India

Summary
Study on genetic diversity was conducted with 30 chilli (Capsicum annuum L.) genotypes of Indian origin at the research farm of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, India during March-October 2011. Twelve quantitative characters among the genotypes for the characters were studied.

Pages 97-102 | Full Text PDF

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Review Article

Scandix pecten-veneris L.: A wild green leafy vegetable

Aglaia Liopa-Tsakalidi

Technological Education Institute of Messolonghi, Department of Mechanical and Water Resources Engineering, Messolonghi, Greece

Summary
Shepherd's needle (Scandix pecten-veneris L.) is an edible wild green leafy vegetable widely used in the Mediterranean diet. It is an archaeophyte, native of Eurasia, dicot, annual scapose herb. S. pecten-veneris is a species which grows in arable land and waste places and prospers in sandy, loam, clay soils. The aim of this review is to provide all the disseminated information regarding the rare or even extinct plant S. pecten veneris, in order to orientate future research.


Pages 103-108 | Full Text PDF
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Desiccation and topping induced silencing of putrescine N-methyl transferase2 regulate nicotine biosynthesis in Nicotiana tabacum cv. Petite Havana

Rex Arunraj, Bindu Sree G*, Madasamy Parani

Department of Genetic Engineering, SRM University, Kattankulathur - 603 203, TamilNadu, India

Summary
The current study was an attempt to understand the regulation of nicotine biosynthesis due to desiccation and meristem topping in Nicotiana tabacum cv. Petite Havana. Our results showed that desiccation induced silencing of
Putrescine- N-Methyl Transferase2 (pmt2) (EC 2.1.1.53), a rate limiting enzyme in the nicotine biosynthetic pathway, regulates nicotine levels in tobacco.

Pages 109-118 | Full Text PDF
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Identification of microsatellite markers for fingerprinting popular Indian flax (Linum usitatissimum L.) cultivars and their utilization in seed genetic purity assessments

Vikas Pali*, Sunil Kumar Verma, Mary Suchita Xalxo, Ritu Ravi Saxena, Nandan Mehta and Satish Balkrishna Verulkar

Department of Genetics and Plant Breeding, College of Agriculture, Krishak Nagar, Indira Gandhi Agricultural University, Raipur 492 012, C. G. India
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Krishak Nagar, Indira Gandhi Agricultural University, Raipur 492 012, C. G. India

Summary
Fingerprinting with molecular markers allows precise and rapid variety identification. The present investigation was carried out with the following objectives: (i) to identify informative SSR marker allele(s) for fingerprinting popular flax cultivars cultivated in Chhattisgarh state of India, and (ii) to validate the utility of the genotype specific SSR markers in seed genetic purity assessments.

Pages 119-126 | Full Text PDF

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Effect of GA3 and nitrogen on yield and marketability of lettuce (Lactuca sativa L.)

George Tsiakaras, Spyridon A. Petropoulos*, Ebrahim M. Khah

Laboratory of Genetics and plant Breeding, Department of Agriculture, CropProduction and Rural Environment, University of Thessaly, Fytokou Str. Nea Ionia 38446, Volos, Greece
Laboratory of Vegetable Production, Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Fytokou Str. Nea Ionia 38446, Volos, Greece

Summary
In the present study the effect of both nitrogen application rate and gibberellic acid (GA3) on yield and earliness of production and marketability (plant height and leaf color) of lettuce was examined. Three commercial cultivars [cv. ‘Kismy’ and ‘Marady’ (loose-leaf types) and cv. ‘Adranita’ (romaine type)] were cultivated in an unheated plastic greenhouse for three consecutive growing periods.

Pages 127-132 | Full Text PDF

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Estimation of genetic parameters and chromosomal localization of QTLs controlling agro-physiological indicators of drought tolerance in agropyron using wheat-agropyron disomic addition lines

Ezatollah Farshadfar*, Shima Rahmani, Mohammad Mahdi
Jowkar, Akbar Shabani

Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
Department of Agronomy and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
Department of Legume, Dryland Agricultural Research Sub-Institute, Kermanshah, Iran

Summary
In order to locate QTLs controlling agro-physiological indicators of drought tolerance and estimation of genetic parameters, disomic chromosome addition lines of Agropyron elongatum (donor) into the genetic background of Chinese Spring (recipient) were tested in the field under rainfed condition.

Pages 133-139 | Full Text PDF

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Rapid separation and identification of wheat HMW glutenin subunits by UPLC and comparative analysis with HPLC

Xing Yan, Wan Liu, Zitong Yu, Caixia Han, Friedrich J. Zeller, Sai L. K. Hsam and Yueming Yan*

College of Life Science, Capital Normal University, Beijing 100048, China
Division of Plant Breeding and Applied Genetics, Technical University of Munich, D-85354 Freising, Germany

Summary
High molecular weight glutenin subunits (HMW-GS) are among the major determinant on wheat bread-making quality. Rapid ultra-performance liquid chromatography (UPLC) methods with high resolution and reproducibility for HMW-GS separation were established in this work. A sample could be completely analyzed in less than 12 min by using the optimized UPLC conditions.

Pages 140-147 | Full Text PDF | Supplementary data PDF
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Morphological and molecular characters: Congruence or conflict in the phylogeny of Sulla species?

Sonia Marghali, Nadia Zitouna, Maroua Gharbi, Houda Chennaoui-Kourda and Neila Trifi-Farah*

Laboratory of Molecular Genetics, Immunology, and Biotechnology, Faculty of Sciences, University of Tunis El Manar, Campus Universitaire, 2092 El Manar, Tunis, Tunisia

Summary
Sulla genus (Hedysarea) includes six Mediterranean species constituting an important phytogenetic patrimony primarily used to promote feed production chiefly in arid and semi-arid regions.  In order to perform a phylogenetic analysis of Sulla species
, both morphological and molecular data were used.

Pages 148-158 | Full Text PDF



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